...PARTIAL CHROMATOGRAPHY OF BETA GALACTOSIDASE BY GEL FILTRATION
PARTIAL CHROMATOGRAPHY OF BETA GALACTOSIDASE BY GEL FILTRATION FROM HUMAN PLACENTA
Mohammad Aberomand1,2, Sareh Aberomand, Kosar Aberomand , Najmah Gholestan Bagh, Sanaz Jevadnia, Mohammad AliMohammadi and Mehnosh Zakerhosini
1- Department of Biochemistry, Ahwaz Jondi Shapur University of Medical Sciences, Ahwaz, Iran.
2- Students Research Committees, Ahwaz Jondishapur University of Medical Sciences, Ahwaz, Iran.
e-mail : aberumand@yahoo.com
(Accepted 28 October 2011)
ABSTRACT – Beta-galactosidases (beta-D-galactoside-galactohydrolases, lactases) (EC:3.2.1.23) are enzymes found in most living beings such as plants, bacteria, fungi and animals as well as human placenta. Beta-galactosidase is important industrial enzyme used for the hydrolysis of lactose from milk. In this study, we partially purified three isoenzymes of beta-galactosidases from human placenta. The partial purified human placental enzyme exhibited high activity at 250C. The beta galactosidase activities showed 8 μ/ml , 7μ/ml and 6μ/ml and specific activities obtained 8.05 μ/mg, 83.66 μ/mg and 24.27 μ/mg respectively. These results suggest that these fractions are three enzymes with difference structure.
Key Words: Beta-galactosidase, human placenta, chromatography.
لینک دانلود ابستراکت مقاله:
۱- http://www.connectjournals.com/file_html_pdf/1216702H_A27.pdf
۲- http://s3.picofile.com/file/7412433331/beta_abstract.pdf.html
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http://s3.picofile.com/file/7548617846/WWW_LABWORLD_IR_beta_g2.jpg
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